ABOUT US
Our Story
Behind the Cells
MethusBio is a trusted purveyor of immunomodulating biologics products internationally. Partnering with industry-leading labs and using gold-standard testing procedures to ensure our products meet the highest standards of quality, consistency and scientific integrity is never compromised by unreliable suppliers or unverifiable claims.
Mission
To accelerate the biologics industry by applying the most recent advancements in cryopreservation, cellular biology, AI, and Web3 technologies.
Vision
Building the early foundation of a rapidly expanding regenerative biologics research industry, as early innovators in an ever-evolving regulatory and scientific environment.
Value Proposition
Bridging ethical sourcing and uncompromised scientific performance.
Rigorous Testing: Every product is subjected to advanced, multi-point quality screening to ensure reliability, consistency, and compatibility with high-precision research protocols.
Why We're Different
Our Commitment to Quality
Random, Unannounced Testing: Vials are tested without warning. If a lab's "validated" batch fails, we don't distribute their products.
Real Post-Thaw Viability: Thawed and tested at final destination a true measurement.
Licensing & Due Diligence: We check every lab's paperwork, equipment, and compliance with international standards. .
Proper Cold Chain Logistics: We maintain LN2 temperatures (< -150c at all times), so the content remains vitrified, tracking and logging the chain of custody.
Our Products
Umbilical-Cord Derived Cell Products
Research Use Only (RUO)
- Passage 3
- Certified pathogen and contaminant-free
- Xeno-free and low immunogenicity
Mesenchymal stromal cells (MSCs), sometimes informally termed "stem cells," are multipotent cells with the ability to differentiate into osteoblasts, chondrocytes, and adipocytes. These cells exhibit a fibroblast-like morphology and adhere to standard tissue culture plastic.
- Surface markers: CD105, CD73, CD90
- Negative for: CD45, CD34, CD14, CD19, HLA-DR
- Characterization method: Flow cytometry
- Research interest: Paracrine signaling and immunomodulation, particularly in autoimmune disease models
Chondrocytes are the sole cellular component of cartilage, responsible for producing and maintaining the extracellular matrix, including collagen and proteoglycans. Derived from mesenchymal stem cells, chondrocytes play a pivotal role in cartilage homeostasis and regeneration research.
- Surface markers: CD44, CD49c, CD151
- Characterization method: Flow cytometry to assess chondrogenic potential
- Research interest: Cartilage repair, osteoarthritis, and regenerative medicine
Neural stem cells (NSCs) are multipotent progenitor cells capable of self-renewal and differentiation into neurons, astrocytes, and oligodendrocytes. These cells are central to research involving neurodevelopment and neurological disorders.
- Markers: Nestin, Sox2, CD133
Nestin: Cytoskeletal protein critical for NSC maintenance
CD133: Surface marker for neural progenitor enrichment
Sox2: Transcription factor essential for pluripotency - Characterization method: Flow cytometry
- Research interest: Neurodegeneration, CNS injury, and cell fate studies
Cardiomyocytes are contractile muscle cells fundamental to heart function. These cells exhibit spontaneous beating activity and are used extensively in cardiac research for modeling physiological responses and evaluating potential therapies.
- Markers: Cardiac Troponin T (cTnT), α-actinin
- Functional protein: Connexin 43 (Cx43), for electrical coupling
- Characterization methods: Flow cytometry and immunocytochemistry
- Research interest: Cardiovascular disease modeling, drug screening, and electrophysiology
Renal stem cells are progenitor cells capable of differentiating into various renal cell lineages. These cells are under investigation for their regenerative capacity and potential contribution to renal repair processes.
- Marker: CD133, indicative of renal progenitor status
- Characterization method: Flow cytometry
- Research interest: Renal injury recovery, organ regeneration, and kidney disease modeling
Exosomes are nanometer-scale extracellular vesicles (30–100 nm) secreted by many cell types, including MSCs. They facilitate cell-cell communication via the transfer of RNA, proteins, and lipids. Exosomes do not contain nuclei and are not considered living entities, though they require cryogenic preservation.
- Markers: CD9, CD63, CD81
- Characterization methods: Flow cytometry, Western blot
- Research interest: Regenerative signaling, biomarker discovery, and drug delivery systems
- Cryopreservation Media: Optimized for ultra-low temperature storage of biologics and cells. Validated for compatibility with stem cells and other sensitive cell types.
- Cell Culture Media: Specialized formulations designed to maintain cell viability and support expansion in controlled laboratory environments.
- Biologics Enhancements: Additives such as growth factors, cytokines, and peptides to support advanced cellular research.
- Custom Solutions:
- Bulk Orders: For labs requiring large quantities of consistent research-grade products.
- Trial Programs: Sample-sized offerings of new products for initial testing.
- Consultation Services: Technical guidance and troubleshooting to ensure optimal product performance.
FAQs
Frequently Asked Questions
Independent Post-Thaw Viability Testing – Many labs report viability pre-freeze, but post-thaw performance can be drastically different. We ensure accurate, independent testing.
Flow Cytometry & Biomarker Validation – Labs use internal testing, but without incentives to be honest. We verify passage number, cell phenotype, and viability with third-party flow cytometry.
Cold Chain Tracking & Compliance – Handling mistakes and temperature fluctuations degrade cells. We enforce continuous temperature monitoring and ensure proper logistics.
Risk Mitigation – Stem cell compliance is complex, and improper sourcing has serious legal and safety risks. We help clients navigate these challenges while maintaining ethical sourcing.
Stem cells are shipped cryopreserved in liquid nitrogen vapor phase (≤−150°C) using a validated dry shipper. This maintains cell viability during transit. Package includes:
- Cryobox or cryobag
- Dry shipper with temp logger
- Shipping manifest
Passage number refers to how many times a cell culture has been subcultured or transferred to a new vessel during laboratory expansion. Each time the cells are split and replated to continue growing, the passage number increases (e.g., P0, P1, P2, etc.).
This number matters because it reflects the replicative age of the cells. Over time, high passage numbers can lead to changes in cell behavior, function, and genetic stability. For therapeutic and research use, maintaining a low passage number ensures high-quality, functional, and consistent cells.
Early-Passage MSCs (P1–P4):
- High proliferation potential
- Low senescence
- Strong differentiation capacity
- Potent immunomodulatory function
Mid-Passage MSCs (P5–P6):
- Decline in proliferation
- Emerging senescence markers
- Reduction in therapeutic potency
Late-Passage MSCs (P7+):
- Reduced differentiation ability
- Weakened immunomodulatory effects
- Increased risk of genetic instability
We use liquid nitrogen (LN2) to keep hUC-MSCs well below the glass phase, or vitrification transition zone, at −150°C, preventing ice crystal formation and metabolic activity. This preserves cell viability, potency, and regulatory compliance during storage and shipping.